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WB analysis of human brain (cerebellum) lysate using GTX88995 RPGRIP1L antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human brain (cerebellum) lysate using GTX88995 RPGRIP1L antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human brain (cerebellum) lysate using GTX88995 RPGRIP1L antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

RPGRIP1L antibody, Internal

GTX88995
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRPGRIP1L
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPGRIP1L antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.3-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23322
  • Target name
    RPGRIP1L
  • Target description
    RPGRIP1 like
  • Target synonyms
    COACH3, CORS3, FTM, JBTS7, MKS5, NPHP8, PPP1R134, protein fantom, RPGR-interacting protein 1-like protein, fantom homolog, nephrocystin-8, protein phosphatase 1, regulatory subunit 134
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ68CZ1
  • Protein Name
    Protein fantom
  • Scientific Description
    The protein encoded by this gene can localize to the basal body-centrosome complex or to primary cilia and centrosomes in ciliated cells. The encoded protein has been found to interact with nephrocystin-4. Defects in this gene are a cause of Joubert syndrome type 7 (JBTS7) and Meckel syndrome type 5 (MKS5). [provided by RefSeq, Jun 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPGRIP1L using anti-RPGRIP1L antibody (A04178-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGRIP1L antigen affinity purified polyclonal antibody (Catalog # A04178-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGRIP1L at approximately 151 kDa. The expected band size for RPGRIP1L is at 151 kDa.
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