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Immunohistochemical staining of human lymph node shows strong membranous positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows strong membranous positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows strong membranous positivity in non-germinal center cells.

Anti-RPS13 Antibody

HPA005985
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRPS13
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS13
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6207
  • Target name
    RPS13
  • Target description
    ribosomal protein S13
  • Target synonyms
    40S ribosomal protein S13; S13; small ribosomal subunit protein uS15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62277
  • Protein Name
    40S ribosomal protein S13
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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WB analysis of various samples using GTX66837 RPS13 antibody. Lane1 : CT26 whole cell lysate(40ug) Lane2 : PC12 whole cell lysate(40ug) Lane3 : MCF-7 whole cell lysate(40ug) Lane4 : A549 whole cell lysate(40ug) Lane5 : HEK293T whole cell lysate(40ug) Lane6 : SGC7901 whole cell lysate(40ug) Dilution : 1:500
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Figure 1. Western blot analysis of RPS13 using anti-RPS13 antibody (A06221-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS13 antigen affinity purified polyclonal antibody (Catalog # A06221-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS13 at approximately 17 kDa. The expected band size for RPS13 is at 17 kDa.
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Western blot All lanes: RPS13 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa
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