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Anti-RPS4X Antibody

144-06730
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRPS4X
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RPS4X Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6191
  • Target name
    RPS4X
  • Target description
    ribosomal protein S4 X-linked
  • Target synonyms
    CCG2, DXS306, RPS4, S4, SCAR, SCR10, eS4, small ribosomal subunit protein eS4, X isoform, 40S ribosomal protein S4, cell cycle gene 2, single copy abundant mRNA protein, single-copy abundant mRNA, small ribosomal subunit protein eS4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62701
  • Protein Name
    Small ribosomal subunit protein eS4, X isoform
  • Scientific Description
    RPS4X Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS4/X/Y1/Y2 using anti-RPS4/X/Y1/Y2 antibody (A09096-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO 320 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS4/X/Y1/Y2 antigen affinity purified polyclonal antibody (Catalog # A09096-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS4/X/Y1/Y2 at approximately 30 kDa. The expected band size for RPS4/X/Y1/Y2 is at 30 kDa.
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