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Western Blot analysis of COLO205 cells using Ribosomal Protein S4X Polyclonal Antibody
Western Blot analysis of COLO205 cells using Ribosomal Protein S4X Polyclonal Antibody
Western Blot analysis of COLO205 cells using Ribosomal Protein S4X Polyclonal Antibody

RPS4X Antibody

CSB-PA004003
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetRPS4X
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPS4X Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6191
  • Target name
    RPS4X
  • Target description
    ribosomal protein S4 X-linked
  • Target synonyms
    40S ribosomal protein S4, X isoform; CCG2; cell cycle gene 2; DXS306; RPS4; S4; SCAR; SCR10; single copy abundant mRNA protein; single-copy abundant mRNA; small ribosomal subunit protein eS4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62701
  • Protein Name
    40S ribosomal protein S4, X isoform
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS4/X/Y1/Y2 using anti-RPS4/X/Y1/Y2 antibody (A09096-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO 320 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS4/X/Y1/Y2 antigen affinity purified polyclonal antibody (Catalog # A09096-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS4/X/Y1/Y2 at approximately 30 kDa. The expected band size for RPS4/X/Y1/Y2 is at 30 kDa.
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