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Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-RPS6KA2 Antibody

HPA045061
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRPS6KA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS6KA2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6196
  • Target name
    RPS6KA2
  • Target description
    ribosomal protein S6 kinase A2
  • Target synonyms
    HU-2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAPK1C; mitogen-activated protein kinase-activated protein kinase 1C; p90RSK2; p90-RSK3; pp90RSK3; ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal S6 kinase 3; RSK; RSK3; S6K-alpha; S6K-alpha2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15349
  • Protein Name
    Ribosomal protein S6 kinase alpha-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RSK3 using anti-RSK3 antibody (A06142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysate, Lane 3: human PC-3 whole cell lysate, Lane 4: human A431 whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human PANC-1 whole cell lysate, Lane 7: rat testis tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody (Catalog # A06142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.
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