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ProductsBack/Anti-RPS6KA3 Antibody
Immunohistochemical staining of human kidney shows nuclear positivity in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows nuclear positivity in cells in tubules and cells in glomeruli.
Immunohistochemical staining of human kidney shows nuclear positivity in cells in tubules and cells in glomeruli.

Anti-RPS6KA3 Antibody

HPA003221
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPS6KA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS6KA3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6197
  • Target name
    RPS6KA3
  • Target description
    ribosomal protein S6 kinase A3
  • Target synonyms
    CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, XLID19, p90-RSK2, pp90RSK2, ribosomal protein S6 kinase alpha-3, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, RSK-2, S6K-alpha-3, epididymis secretory sperm binding protein, insulin-stimulated protein kinase 1, p90-RSK 3, ribosomal S6 kinase 2, ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51812
  • Protein Name
    Ribosomal protein S6 kinase alpha-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.
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