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RPS6KA3 Polyclonal Antibody

CAC15060
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRPS6KA3
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Overview

  • Supplier
    Biomatik
  • Product Name
    RPS6KA3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6197
  • Target name
    RPS6KA3
  • Target description
    ribosomal protein S6 kinase A3
  • Target synonyms
    CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, XLID19, p90-RSK2, pp90RSK2, ribosomal protein S6 kinase alpha-3, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, RSK-2, S6K-alpha-3, epididymis secretory sperm binding protein, insulin-stimulated protein kinase 1, p90-RSK 3, ribosomal S6 kinase 2, ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51812
  • Protein Name
    Ribosomal protein S6 kinase alpha-3
  • Scientific Description
    The RPS6KA3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.
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