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Immunohistochemical staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-RPS6KA4 Antibody

HPA023225
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPS6KA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPS6KA4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8986
  • Target name
    RPS6KA4
  • Target description
    ribosomal protein S6 kinase A4
  • Target synonyms
    MSK2, RSK-B, S6K-alpha-4, ribosomal protein S6 kinase alpha-4, 90 kDa ribosomal protein S6 kinase 4, mitogen- and stress-activated protein kinase 2, nuclear mitogen- and stress-activated protein kinase 2, ribosomal protein S6 kinase, 90kDa, polypeptide 4, ribosomal protein kinase B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75676
  • Protein Name
    Ribosomal protein S6 kinase alpha-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 antigen affinity purified polyclonal antibody (Catalog # A05545-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK2/RSK-B/RPS6KA4 at approximately 86 kDa. The expected band size for MSK2/RSK-B/RPS6KA4 is at 86 kDa.
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