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ProductsBack/RPS6KA4 / MSK2 / RSK-B Antibody

RPS6KA4 / MSK2 / RSK-B Antibody

LS-C496842
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRPS6KA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPS6KA4 / MSK2 / RSK-B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 84kDa/85kDa, while the observed MW by Western blot was 115kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:1000) The predicted MW is 84kDa/85kDa, while the observed MW by Western blot was 115kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8986
  • Target name
    RPS6KA4
  • Target description
    ribosomal protein S6 kinase A4
  • Target synonyms
    MSK2, RSK-B, S6K-alpha-4, ribosomal protein S6 kinase alpha-4, 90 kDa ribosomal protein S6 kinase 4, mitogen- and stress-activated protein kinase 2, nuclear mitogen- and stress-activated protein kinase 2, ribosomal protein S6 kinase, 90kDa, polypeptide 4, ribosomal protein kinase B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 antigen affinity purified polyclonal antibody (Catalog # A05545-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK2/RSK-B/RPS6KA4 at approximately 86 kDa. The expected band size for MSK2/RSK-B/RPS6KA4 is at 86 kDa.
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