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Anti-RRM2 [SAIC-30C-18]

AB00322-1.1-BT
Absolute Antibody
ApplicationsMass Spectrometry, Western Blot, ELISA
Product group Antibodies
TargetRRM2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-RRM2 [SAIC-30C-18]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-30C-18
  • Gene ID6241
  • Target name
    RRM2
  • Target description
    ribonucleotide reductase regulatory subunit M2
  • Target synonyms
    C2orf48, R2, RR2, RR2M, ribonucleoside-diphosphate reductase subunit M2, ribonucleotide reductase M2 polypeptide, ribonucleotide reductase small chain, ribonucleotide reductase small subunit, uncharacterized protein C2orf48
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31350
  • Protein Name
    Ribonucleoside-diphosphate reductase subunit M2
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRM2 using anti-RRM2 antibody (PB9817). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug, Lane 3: A431 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified polyclonal antibody (Catalog # PB9817) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 50 kDa. The expected band size for RRM2 is at 50 kDa.
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