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Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Anti-RRP8 Antibody

HPA038487
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRRP8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RRP8
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23378
  • Target name
    RRP8
  • Target description
    ribosomal RNA processing 8
  • Target synonyms
    cerebral protein 1; KIAA0409; NML; nucleomethylin; ribosomal RNA processing 8, methyltransferase, homolog; ribosomal RNA-processing protein 8; RRP8 methyltransferase homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43159
  • Protein Name
    Ribosomal RNA-processing protein 8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP8 using anti-RRP8 antibody (A09950-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP8 antigen affinity purified polyclonal antibody (Catalog # A09950-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP8 at approximately 65 kDa. The expected band size for RRP8 is at 51 kDa.
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