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Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Anti-RSPH3 Antibody

HPA039109
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRSPH3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RSPH3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83861
  • Target name
    RSPH3
  • Target description
    radial spoke head 3
  • Target synonyms
    CILD32, RSHL2, RSP3, dJ111C20.1, radial spoke head protein 3 homolog, A-kinase anchor protein RSPH3, radial spoke 3 homolog, radial spoke head 3 homolog, radial spoke head-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UC2
  • Protein Name
    Radial spoke head protein 3 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RSPH3 using anti-RSPH3 antibody (A11829). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Siha whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSPH3 antigen affinity purified polyclonal antibody (Catalog # A11829) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSPH3 at approximately 70 kDa. The expected band size for RSPH3 is at 64 kDa.
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TargetRSPH3
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