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RSPH3 Antibody

CSB-PA769775LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetRSPH3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RSPH3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83861
  • Target name
    RSPH3
  • Target description
    radial spoke head 3
  • Target synonyms
    CILD32, RSHL2, RSP3, dJ111C20.1, radial spoke head protein 3 homolog, A-kinase anchor protein RSPH3, radial spoke 3 homolog, radial spoke head 3 homolog, radial spoke head-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UC2
  • Protein Name
    Radial spoke head protein 3 homolog
  • Scientific Description
    Functions as a protein kinase A-anchoring protein that scaffolds the cAMP-dependent protein kinase holoenzyme. May serve as a point of convergence for MAPK and PKA signaling in cilia.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RSPH3 using anti-RSPH3 antibody (A11829). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Siha whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSPH3 antigen affinity purified polyclonal antibody (Catalog # A11829) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSPH3 at approximately 70 kDa. The expected band size for RSPH3 is at 64 kDa.
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