Anti-RSPO1 [89M5]

Absolute Antibody

Anti-RSPO1 [89M5]

9

The antibody specifically binds human RSPO1. The antibody cross-reacts with mouse RSPO1

The binding affinity of the original format of the antibody to human and mouse RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM respectively). Further, the binding affinity of the humanized version of the antibody, h89M5-H2L2, to human RSPO1 was measured by surface plasmon resonance (Kd less than 0.1 nM). The original format of the antibody could inhibit tumor cell growth in vivo in an ovarian tumor xenograft model as single agents and in combination with a chemotherapeutic agent. The original format of the antibody could inhibit tumor cell growth in vivo in a pancreatic tumor xenograft model in combination with a chemotherapeutic agent. The original antibody was able to block RSPO1-induced beta-catenin signaling in HEK-293 cells. The original antibody blocked binding of RSPO1 to LGR5 in HEK-293 cells, as measured by FACS. The original and humanized format of the antibody, could bind to human RSPO1 expressed on HEK-293 cells, as shown by FACS analysis (US8802097B2).

Flow Cytometry, Other Application

Research Use Only

Monoclonal

89M5

RSPO1

R-spondin 1

CRISTIN3; roof plate-specific spondin-1; RSPO; R-spondin homolog; R-spondin-1

Human

IgG1

R-spondin-1

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

-20°C,2°C to 8°C

12352203