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Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Anti-RSRC2 Antibody

HPA040070
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRSRC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RSRC2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65117
  • Target name
    RSRC2
  • Target description
    arginine and serine rich coiled-coil 2
  • Target synonyms
    arginine/serine-rich coiled-coil 2; arginine/serine-rich coiled-coil protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L4I2
  • Protein Name
    Arginine/serine-rich coiled-coil protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: Large intestine lysates probed with RSRC2 Polyclonal Antibody, Unconjugated (bs-6097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
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Figure 1. Western blot analysis of RSRC2 using anti-RSRC2 antibody (A13417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSRC2 antigen affinity purified polyclonal antibody (Catalog # A13417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSRC2 at approximately 58 kDa. The expected band size for RSRC2 is at 51 kDa.
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