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Immunohistochemical staining of human placenta shows moderate membranous positivity in endothelial cells.
Immunohistochemical staining of human placenta shows moderate membranous positivity in endothelial cells.
Immunohistochemical staining of human placenta shows moderate membranous positivity in endothelial cells.

Anti-RTL1 Antibody

HPA077601
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRTL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RTL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID388015
  • Target name
    RTL1
  • Target description
    retrotransposon Gag like 1
  • Target synonyms
    HUR1, MART1, Mar1, PEG11, SIRH2, retrotransposon-like protein 1, Sushi-Ichi retrotransposon homolog 2, mammalian retrotransposon derived protein 1, mammalian retrotransposon-derived 1, paternally expressed 11, paternally expressed gene 11 protein, retrotransposon-derived protein PEG11, retrotransposon-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA6NKG5
  • Protein Name
    Retrotransposon-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RTL1 using anti-RTL1 antibody (A11807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL1 antigen affinity purified polyclonal antibody (Catalog # A11807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTL1 at approximately 200 kDa. The expected band size for RTL1 is at 155 kDa.
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