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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.

Anti-RUFY2 Antibody

HPA039792
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRUFY2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RUFY2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55680
  • Target name
    RUFY2
  • Target description
    RUN and FYVE domain containing 2
  • Target synonyms
    RABIP4R, ZFYVE13, RUN and FYVE domain-containing protein 2, Run- and FYVE-domain containing protein, antigen MU-RMS-40.17, rab4-interacting protein related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXA3
  • Protein Name
    RUN and FYVE domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Western blot All lanes: RUFY2 antibody at 1.69ug/ml Lane 1: PC-3 whole cell lysate Lane 2: Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 71, 75, 47, 44 kDa Observed band size: 76 kDa
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Figure 1. Western blot analysis of RUFY2 using anti-RUFY2 antibody (A13178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY2 antigen affinity purified polyclonal antibody (Catalog # A13178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY2 at approximately 69 kDa. The expected band size for RUFY2 is at 65-70 kDa.
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