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Western blot All lanes: RUFY2 antibody at 1.69ug/ml Lane 1: PC-3 whole cell lysate Lane 2: Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 71, 75, 47, 44 kDa Observed band size: 76 kDa
Western blot All lanes: RUFY2 antibody at 1.69ug/ml Lane 1: PC-3 whole cell lysate Lane 2: Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 71, 75, 47, 44 kDa Observed band size: 76 kDa
Western blot All lanes: RUFY2 antibody at 1.69ug/ml Lane 1: PC-3 whole cell lysate Lane 2: Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 71, 75, 47, 44 kDa Observed band size: 76 kDa

RUFY2 Antibody

CSB-PA848835ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRUFY2
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Overview

  • Supplier
    Cusabio
  • Product Name
    RUFY2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55680
  • Target name
    RUFY2
  • Target description
    RUN and FYVE domain containing 2
  • Target synonyms
    RABIP4R, ZFYVE13, RUN and FYVE domain-containing protein 2, Run- and FYVE-domain containing protein, antigen MU-RMS-40.17, rab4-interacting protein related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXA3
  • Protein Name
    RUN and FYVE domain-containing protein 2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RUFY2 using anti-RUFY2 antibody (A13178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY2 antigen affinity purified polyclonal antibody (Catalog # A13178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY2 at approximately 69 kDa. The expected band size for RUFY2 is at 65-70 kDa.
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