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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Anti-RUFY3 Antibody

HPA022970
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRUFY3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RUFY3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22902
  • Target name
    RUFY3
  • Target description
    RUN and FYVE domain containing 3
  • Target synonyms
    RIPX, SINGAR1, ZFYVE30, protein RUFY3, rap2 interacting protein x, single axon-regulated protein, single axon-related 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L099
  • Protein Name
    Protein RUFY3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RUFY3 using anti-RUFY3 antibody (A09453-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY3 antigen affinity purified polyclonal antibody (Catalog # A09453-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY3 at approximately 53-65 kDa. The expected band size for RUFY3 is at 53-65 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa
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