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Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: RUFY3 antibody at 3.71microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 56, 71, 65 kDa Observed band size: 53 kDa

RUFY3 Antibody

CSB-PA765974LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRUFY3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RUFY3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22902
  • Target name
    RUFY3
  • Target description
    RUN and FYVE domain containing 3
  • Target synonyms
    RIPX, SINGAR1, ZFYVE30, protein RUFY3, rap2 interacting protein x, single axon-regulated protein, single axon-related 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L099
  • Protein Name
    Protein RUFY3
  • Scientific Description
    Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (PubMed:25766321). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (PubMed:25766321).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RUFY3 using anti-RUFY3 antibody (A09453-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY3 antigen affinity purified polyclonal antibody (Catalog # A09453-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY3 at approximately 53-65 kDa. The expected band size for RUFY3 is at 53-65 kDa.
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