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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.

Anti-S100A6 Antibody

HPA007575
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetS100A6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-S100A6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6277
  • Target name
    S100A6
  • Target description
    S100 calcium binding protein A6
  • Target synonyms
    2A9; 5B10; CABP; CACY; calcyclin; growth factor-inducible protein 2A9; MLN 4; PRA; prolactin receptor-associated protein; protein S100-A6; S10A6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06703
  • Protein Name
    Protein S100-A6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of S100A6 using anti-S100A6 antibody (PB9676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 antigen affinity purified polyclonal antibody (Catalog # PB9676) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A6 at approximately 10 kDa. The expected band size for S100A6 is at 10 kDa.
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