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ProductsBack/S100A6 Recombinant Antibody, AbBy Fluor-350 Conjugated

S100A6 Recombinant Antibody, AbBy Fluor-350 Conjugated

BSM-61393R-BF350
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetS100A6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    S100A6 Recombinant Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID6277
  • Target name
    S100A6
  • Target description
    S100 calcium binding protein A6
  • Target synonyms
    2A9; 5B10; CABP; CACY; calcyclin; growth factor-inducible protein 2A9; MLN 4; PRA; prolactin receptor-associated protein; protein S100-A6; S10A6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06703
  • Protein Name
    Protein S100-A6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100A6 using anti-S100A6 antibody (PB9676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 antigen affinity purified polyclonal antibody (Catalog # PB9676) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A6 at approximately 10 kDa. The expected band size for S100A6 is at 10 kDa.
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