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Immunohistochemical staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.

Anti-SAFB Antibody

HPA016832
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAFB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAFB
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6294
  • Target name
    SAFB
  • Target description
    scaffold attachment factor B
  • Target synonyms
    HAP, HET, SAB-B1, SAF-B, SAF-B1, SAFB1, scaffold attachment factor B1, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, Hsp27 ERE-TATA binding protein, glutathione S-transferase fusion protein, heat-shock protein (HSP27) estrogen response element and TATA box-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15424
  • Protein Name
    Scaffold attachment factor B1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SAFB using anti-SAFB antibody (PB10095). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAFB antigen affinity purified polyclonal antibody (Catalog # PB10095) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAFB at approximately 150 kDa. The expected band size for SAFB is at 103 kDa.
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