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ProductsBack/SAFB1 / SAFB Antibody

SAFB1 / SAFB Antibody

LS-C409474
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSAFB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SAFB1 / SAFB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 95kDa/102kDa, while the observed MW by Western blot was 130kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 95kDa/102kDa, while the observed MW by Western blot was 130kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6294
  • Target name
    SAFB
  • Target description
    scaffold attachment factor B
  • Target synonyms
    glutathione S-transferase fusion protein; HAP; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein; HET; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAB-B1; SAF-B; SAFB1; SAF-B1; scaffold attachment factor B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAFB using anti-SAFB antibody (PB10095). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAFB antigen affinity purified polyclonal antibody (Catalog # PB10095) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAFB at approximately 150 kDa. The expected band size for SAFB is at 103 kDa.
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