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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Anti-SAMSN1 Antibody

HPA010645
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAMSN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAMSN1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64092
  • Target name
    SAMSN1
  • Target description
    SAM domain, SH3 domain and nuclear localization signals 1
  • Target synonyms
    HACS1, NASH1, SASH2, SH3D6B, SLy2, SAM domain-containing protein SAMSN-1, SAM and SH3 domain containing 2, SAM domain, SH3 domain and nuclear localisation signals, 1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein, Src homology domain 3 (SH3)-containing adapter protein SH3 lymphocyte protein 2, hematopoietic adapter-containing SH3 and sterile I+/--motif (SAM) domains 1, hematopoietic adapter-containing SH3 and sterile alpha-motif (SAM) domains 1, hematopoietic adaptor containing SH3 and SAM domains 1, nuclear localization signals, SAM and SH3 domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NSI8
  • Protein Name
    SAM domain-containing protein SAMSN-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMSN1 antigen affinity purified polyclonal antibody (Catalog # A08977-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAMSN1 at approximately 50 kDa. The expected band size for SAMSN1 is at 42 kDa.
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