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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Anti-SARS2 Antibody

HPA056957
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSARS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SARS2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54938
  • Target name
    SARS2
  • Target description
    seryl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    mitochondrial seryl-tRNA synthetase; mtSerRS; SARS; SARSM; serine tRNA ligase 2, mitochondrial; serine--tRNA ligase, mitochondrial; SerRS; SerRSmt; SERS; seryl-tRNA synthetase, mitochondrial; seryl-tRNA(Ser/Sec) synthetase; SYS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP81
  • Protein Name
    Serine--tRNA ligase, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SARS2 using anti-SARS2 antibody (A08425-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARS2 antigen affinity purified polyclonal antibody (Catalog # A08425-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SARS2 at approximately 53 kDa. The expected band size for SARS2 is at 58 kDa.
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