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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Anti-SAV1 Antibody

HPA001808
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSAV1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SAV1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID60485
  • Target name
    SAV1
  • Target description
    salvador family WW domain containing protein 1
  • Target synonyms
    1700040G09Rik; 45 kDa WW domain protein; hWW45; protein salvador homolog 1; salvador homolog 1; SAV; WW domain-containing adaptor 45; WW45; WWP4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4B6
  • Protein Name
    Protein salvador homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate All lanes: SAV1 antibody at 3.64microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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WB analysis of various sample lysates using GTX66596 Salvador homolog 1 antibody. Dilution : 1:1000 Loading : 25microg per lane
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Figure 1. Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.
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