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Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-SCARA5 Antibody

HPA024661
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSCARA5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCARA5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID286133
  • Target name
    SCARA5
  • Target description
    scavenger receptor class A member 5
  • Target synonyms
    NET33, Tesr, scavenger receptor class A member 5, scavenger receptor class A, member 5 (putative), scavenger receptor hlg, testis expressed scavenger receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMJ2
  • Protein Name
    Scavenger receptor class A member 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCARA5 using anti-SCARA5 antibody (A09571-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARA5 antigen affinity purified polyclonal antibody (Catalog # A09571-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARA5 at approximately 50 kDa. The expected band size for SCARA5 is at 54 kDa.
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