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WB analysis of human liver tissue lysate using GTX85270 SCARA5 antibody. Working concentration : 1 microg/ml
WB analysis of human liver tissue lysate using GTX85270 SCARA5 antibody. Working concentration : 1 microg/ml
WB analysis of human liver tissue lysate using GTX85270 SCARA5 antibody. Working concentration : 1 microg/ml

SCARA5 antibody

GTX85270
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetSCARA5
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCARA5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. ICC/IF: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID286133
  • Target name
    SCARA5
  • Target description
    scavenger receptor class A member 5
  • Target synonyms
    NET33, Tesr, scavenger receptor class A member 5, scavenger receptor class A, member 5 (putative), scavenger receptor hlg, testis expressed scavenger receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMJ2
  • Protein Name
    Scavenger receptor class A member 5
  • Scientific Description
    Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains that collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery. At least four isoforms of SCARB1 are known to exist.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • TGF-beta1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB. Huang SC et al., 2017 Dec, EMBO Mol Med
    Read more

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Figure 1. Western blot analysis of SCARA5 using anti-SCARA5 antibody (A09571-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARA5 antigen affinity purified polyclonal antibody (Catalog # A09571-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARA5 at approximately 50 kDa. The expected band size for SCARA5 is at 54 kDa.
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