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Anti-SCN1B Antibody

144-10071
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSCN1B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SCN1B Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6324
  • Target name
    SCN1B
  • Target description
    sodium voltage-gated channel beta subunit 1
  • Target synonyms
    ATFB13; BRGDA5; DEE52; EIEE52; GEFSP1; sodium channel subunit beta-1; sodium channel, voltage gated, type I beta subunit; sodium channel, voltage-gated, type I, beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07699
  • Protein Name
    Sodium channel subunit beta-1
  • Scientific Description
    SCN1B Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCN1B using anti-SCN1B antibody (A03061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat hippocampus tissue lysates, Lane 2: mouse hippocampus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN1B antigen affinity purified polyclonal antibody (Catalog # A03061-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN1B at approximately 25 kDa, 38kDa. The expected band size for SCN1B is at 25 kDa.
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IHC image of CSB-PA020835LA01HU diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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