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ProductsBack/SCN1B antibody, Internal
WB analysis of HeLa cells using GTX47701 SCN1B antibody at 0.2-1microg/ml.
WB analysis of HeLa cells using GTX47701 SCN1B antibody at 0.2-1microg/ml.
WB analysis of HeLa cells using GTX47701 SCN1B antibody at 0.2-1microg/ml.

SCN1B antibody, Internal

GTX47701
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSCN1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCN1B antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6324
  • Target name
    SCN1B
  • Target description
    sodium voltage-gated channel beta subunit 1
  • Target synonyms
    ATFB13; BRGDA5; DEE52; EIEE52; GEFSP1; sodium channel subunit beta-1; sodium channel, voltage gated, type I beta subunit; sodium channel, voltage-gated, type I, beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07699
  • Protein Name
    Sodium channel subunit beta-1
  • Scientific Description
    Voltage-gated sodium channels are heteromeric proteins that function in the generation and propagation of action potentials in muscle and neuronal cells. They are composed of one alpha and two beta subunits, where the alpha subunit provides channel activity and the beta-1 subunit modulates the kinetics of channel inactivation. This gene encodes a sodium channel beta-1 subunit. Mutations in this gene result in generalized epilepsy with febrile seizures plus, Brugada syndrome 5, and defects in cardiac conduction. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCN1B using anti-SCN1B antibody (A03061-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat hippocampus tissue lysates, Lane 2: mouse hippocampus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN1B antigen affinity purified polyclonal antibody (Catalog # A03061-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN1B at approximately 25 kDa, 38kDa. The expected band size for SCN1B is at 25 kDa.
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IHC image of CSB-PA020835LA01HU diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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