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Immunofluorescent staining of human cell line MCF7 shows localization to golgi apparatus. Antibody staining is shown in green.
Immunofluorescent staining of human cell line MCF7 shows localization to golgi apparatus. Antibody staining is shown in green.
Immunofluorescent staining of human cell line MCF7 shows localization to golgi apparatus. Antibody staining is shown in green.

Anti-SCYL1 Antibody

HPA015015
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSCYL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCYL1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57410
  • Target name
    SCYL1
  • Target description
    SCY1 like pseudokinase 1
  • Target synonyms
    GKLP, HT019, NKTL, NTKL, P105, SCAR21, TAPK, TEIF, TRAP, N-terminal kinase-like protein, SCY1-like protein 1, SCY1-like, kinase-like 1, coated vesicle-associated kinase of 90 kDa, likely ortholog of mouse N-terminal kinase-like protein, telomerase regulation-associated protein, telomerase transcriptional elements-interacting factor, teratoma-associated tyrosine kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96KG9
  • Protein Name
    N-terminal kinase-like protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SCYL1 using anti-SCYL1 antibody (A04750-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL1 antigen affinity purified polyclonal antibody (Catalog # A04750-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL1 at approximately 115 kDa. The expected band size for SCYL1 is at 90 kDa.
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