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Immunohistochemical staining of human colon shows granular positivity in glandular cells.
Immunohistochemical staining of human colon shows granular positivity in glandular cells.
Immunohistochemical staining of human colon shows granular positivity in glandular cells.

Anti-SCYL2 Antibody

HPA021050
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSCYL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SCYL2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55681
  • Target name
    SCYL2
  • Target description
    SCY1 like pseudokinase 2
  • Target synonyms
    AMC4, AMCNACC, CVAK104, SCY1-like protein 2, SCY1-like 2, SCY1-like, kinase-like 2, coated vesicle-associated kinase of 104 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P3W7
  • Protein Name
    SCY1-like protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SCYL2 using anti-SCYL2 antibody (A08578-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL2 antigen affinity purified polyclonal antibody (Catalog # A08578-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL2 at approximately 104 kDa. The expected band size for SCYL2 is at 104 kDa.
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