ICC/IF analysis of MCF-7 cells using GTX00732 SCYL2 antibody. Green : Primary antibody Blue : DAPI Dilution : 1:1000 Fixation : 4% PFA Permeabilization : 0.25% Triton X-100 in PBS
ICC/IF analysis of MCF-7 cells using GTX00732 SCYL2 antibody. Green : Primary antibody Blue : DAPI Dilution : 1:1000 Fixation : 4% PFA Permeabilization : 0.25% Triton X-100 in PBS
SCYL2 antibody
GTX00732
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSCYL2
Overview
- SupplierGeneTex
- Product NameSCYL2 antibody - KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:200-1:1000. IP: 1:200-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID55681
- Target nameSCYL2
- Target descriptionSCY1 like pseudokinase 2
- Target synonymsAMC4; AMCNACC; coated vesicle-associated kinase of 104 kDa; CVAK104; SCY1-like 2; SCY1-like protein 2; SCY1-like, kinase-like 2
- HostRabbit
- IsotypeIgG
- Protein IDQ6P3W7
- Protein NameSCY1-like protein 2
- Scientific DescriptionThe protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
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