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Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferus ducts.

Anti-SDE2 Antibody

HPA031256
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSDE2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SDE2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID163859
  • Target name
    SDE2
  • Target description
    spliceosome associated SDE2
  • Target synonyms
    C1orf55, dJ671D7.1, splicing regulator SDE2, SDE2 telomere maintenance homolog, UPF0667 protein C1orf55, protein SDE2 homolog, replication stress response regulator SDE2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IQ49
  • Protein Name
    Splicing regulator SDE2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SDE2 using anti-SDE2 antibody (A15473-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDE2 antigen affinity purified polyclonal antibody (Catalog # A15473-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDE2 at approximately 54 kDa. The expected band size for SDE2 is at 50 kDa.
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