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Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in basal layer of squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in basal layer of squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in basal layer of squamous epithelial cells.

Anti-SEC13 Antibody

HPA035292
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEC13
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC13 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6396
  • Target name
    SEC13
  • Target description
    SEC13 homolog, nuclear pore and COPII component
  • Target synonyms
    D3S1231E, SEC13L1, SEC13R, npp-20, protein SEC13 homolog, GATOR complex protein SEC13, GATOR2 complex protein SEC13, SEC13 homolog, nuclear pore and COPII coat complex component, SEC13 homolog, nuclear pore and COPII coating complex component, SEC13-like protein 1, SEC13-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55735
  • Protein Name
    Protein SEC13 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SEC13L1/SEC13 using anti-SEC13L1/SEC13 antibody (A04346-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat small intestine tissue lysates, Lane 7: mosue brain tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC13L1/SEC13 antigen affinity purified polyclonal antibody (Catalog # A04346-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC13L1/SEC13 at approximately 36 kDa. The expected band size for SEC13L1/SEC13 is at 36 kDa.
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