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Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-SEC14L3 Antibody

HPA059450
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEC14L3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC14L3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID266629
  • Target name
    SEC14L3
  • Target description
    SEC14 like lipid binding 3
  • Target synonyms
    TAP2, SEC14-like protein 3, SEC14-like 3, SEC14p-like protein TAP2, tocopherol-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UDX4
  • Protein Name
    SEC14-like protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: Mouse liver tissue, Mouse lung tissue All lanes: SEC14L3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
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Figure 1. Western blot analysis of SEC14L3/TAP2 using anti-SEC14L3/TAP2 antibody (A14501-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC14L3/TAP2 antigen affinity purified polyclonal antibody (Catalog # A14501-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC14L3/TAP2 at approximately 47KD. The expected band size for SEC14L3/TAP2 is at 47KD.
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