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Western Blot Positive WB detected in: Mouse liver tissue, Mouse lung tissue All lanes: SEC14L3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse lung tissue All lanes: SEC14L3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse lung tissue All lanes: SEC14L3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa

SEC14L3 Antibody

CSB-PA887012LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSEC14L3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEC14L3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID266629
  • Target name
    SEC14L3
  • Target description
    SEC14 like lipid binding 3
  • Target synonyms
    TAP2, SEC14-like protein 3, SEC14-like 3, SEC14p-like protein TAP2, tocopherol-associated protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UDX4
  • Protein Name
    SEC14-like protein 3
  • Scientific Description
    Probable hydrophobic ligand-binding protein; may play a role in the transport of hydrophobic ligands like tocopherol, squalene and phospholipids.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEC14L3/TAP2 using anti-SEC14L3/TAP2 antibody (A14501-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC14L3/TAP2 antigen affinity purified polyclonal antibody (Catalog # A14501-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC14L3/TAP2 at approximately 47KD. The expected band size for SEC14L3/TAP2 is at 47KD.
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