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Immunohistochemical staining of human endometrium shows positivit in glandular cells.
Immunohistochemical staining of human endometrium shows positivit in glandular cells.
Immunohistochemical staining of human endometrium shows positivit in glandular cells.

Anti-SEC31A Antibody

HPA005457
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEC31A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC31A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22872
  • Target name
    SEC31A
  • Target description
    SEC31 homolog A, COPII component
  • Target synonyms
    ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1, protein transport protein Sec31A, SEC31 homolog A, COPII coat complex component, SEC31 homolog A, COPII coating complex component, SEC31-like protein 1, SEC31-related protein A, web1-like protein, yeast Sec31p homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94979
  • Protein Name
    Protein transport protein Sec31A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SEC31A using anti-SEC31A antibody (A04582). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC31A antigen affinity purified polyclonal antibody (Catalog # A04582) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC31A at approximately 133 kDa. The expected band size for SEC31A is at 133 kDa.
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