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ProductsBack/SEC31A / HSPC275 Antibody

SEC31A / HSPC275 Antibody

LS-C410855
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEC31A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEC31A / HSPC275 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID22872
  • Target name
    SEC31A
  • Target description
    SEC31 homolog A, COPII component
  • Target synonyms
    ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1, protein transport protein Sec31A, SEC31 homolog A, COPII coat complex component, SEC31 homolog A, COPII coating complex component, SEC31-like protein 1, SEC31-related protein A, web1-like protein, yeast Sec31p homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC31A using anti-SEC31A antibody (A04582). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC31A antigen affinity purified polyclonal antibody (Catalog # A04582) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC31A at approximately 133 kDa. The expected band size for SEC31A is at 133 kDa.
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