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Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-SEPT4 antibody. Corresponding SEPT4 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-SEPT4 antibody. Corresponding SEPT4 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-SEPT4 antibody. Corresponding SEPT4 RNA-seq data are presented for the same tissues.

Anti-SEPT4 Antibody

HPA021587
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSEPTIN4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEPT4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5414
  • Target name
    SEPTIN4
  • Target description
    septin 4
  • Target synonyms
    apoptosis-related protein in the TGF-beta signaling pathway; ARTS; BRADEION; bradeion beta; brain protein H5; C17orf47; CE5B3; CE5B3 beta; cell division control-related protein 2; cerebral protein 7; H5; hCDCREL-2; hucep-7; MART; peanut-like protein 2; PNUTL2; SEP4; SEPT4; septin-4; septin-M; uncharacterized protein C17orf47
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43236
  • Protein Name
    Septin-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEPT4/SEPTIN4 using anti-SEPT4/SEPTIN4 antibody (A09411-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT4/SEPTIN4 antigen affinity purified polyclonal antibody (Catalog # A09411-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT4/SEPTIN4 at approximately 50 kDa. The expected band size for SEPT4/SEPTIN4 is at 50 kDa.
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