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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA113302(SEPT4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA113302(SEPT4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA113302(SEPT4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

SEPT4 Antibody

CSB-PA113302
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSEPTIN4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5414
  • Target name
    SEPTIN4
  • Target description
    septin 4
  • Target synonyms
    apoptosis-related protein in the TGF-beta signaling pathway; ARTS; BRADEION; bradeion beta; brain protein H5; C17orf47; CE5B3; CE5B3 beta; cell division control-related protein 2; cerebral protein 7; H5; hCDCREL-2; hucep-7; MART; peanut-like protein 2; PNUTL2; SEP4; SEPT4; septin-4; septin-M; uncharacterized protein C17orf47
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43236
  • Protein Name
    Septin-4
  • Scientific Description
    This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEPT4/SEPTIN4 using anti-SEPT4/SEPTIN4 antibody (A09411-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT4/SEPTIN4 antigen affinity purified polyclonal antibody (Catalog # A09411-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT4/SEPTIN4 at approximately 50 kDa. The expected band size for SEPT4/SEPTIN4 is at 50 kDa.
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