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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.

Anti-SERPINC1 Antibody

HPA001816
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetSERPINC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SERPINC1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID462
  • Target name
    SERPINC1
  • Target description
    serpin family C member 1
  • Target synonyms
    antithrombin-III; AT3; AT3D; ATIII; ATIII-R2; ATIII-T1; ATIII-T2; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serpin peptidase inhibitor clade C member 1; serpin peptidase inhibitor, clade C (antithrombin), member 1; THPH7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01008
  • Protein Name
    Antithrombin-III
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Antithrombin III/SERPINC1 using anti-Antithrombin III/SERPINC1 antibody (A00469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Antithrombin III/SERPINC1 antigen affinity purified polyclonal antibody (Catalog # A00469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Antithrombin III/SERPINC1 at approximately 53 kDa. The expected band size for Antithrombin III/SERPINC1 is at 52 kDa.
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