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Immunohistochemical staining of human colon shows distinctly stained plasma.
Immunohistochemical staining of human colon shows distinctly stained plasma.
Immunohistochemical staining of human colon shows distinctly stained plasma.

Anti-SERPIND1 Antibody

HPA055767
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSERPIND1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SERPIND1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3053
  • Target name
    SERPIND1
  • Target description
    serpin family D member 1
  • Target synonyms
    D22S673; HC2; HCF2; HCII; heparin cofactor 2; heparin cofactor II splice isoform; HLS2; leuserpin 2; LS2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; THPH10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05546
  • Protein Name
    Heparin cofactor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPIND1 using anti-SERPIND1 antibody (A04607-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPIND1 antigen affinity purified polyclonal antibody (Catalog # A04607-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPIND1 at approximately 70 kDa. The expected band size for SERPIND1 is at 57-60,66-75 kDa.
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ICC/IF analysis of HepG2 cells using GTX87285 Heparin Cofactor II antibody. The picture on the right is blocked with the synthesized peptide.
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