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ICC/IF analysis of HepG2 cells using GTX87285 Heparin Cofactor II antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87285 Heparin Cofactor II antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of HepG2 cells using GTX87285 Heparin Cofactor II antibody. The picture on the right is blocked with the synthesized peptide.

Heparin Cofactor II antibody

GTX87285
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSERPIND1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Heparin Cofactor II antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100~1:500. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3053
  • Target name
    SERPIND1
  • Target description
    serpin family D member 1
  • Target synonyms
    D22S673; HC2; HCF2; HCII; heparin cofactor 2; heparin cofactor II splice isoform; HLS2; leuserpin 2; LS2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; THPH10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05546
  • Protein Name
    Heparin cofactor 2
  • Scientific Description
    This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPIND1 using anti-SERPIND1 antibody (A04607-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPIND1 antigen affinity purified polyclonal antibody (Catalog # A04607-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPIND1 at approximately 70 kDa. The expected band size for SERPIND1 is at 57-60,66-75 kDa.
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