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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.

Anti-SESN1 Antibody

HPA073659
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSESN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SESN1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27244
  • Target name
    SESN1
  • Target description
    sestrin 1
  • Target synonyms
    PA26, SEST1, sestrin-1, p53 activated gene 26, p53 regulated PA26 nuclear protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6P5
  • Protein Name
    Sestrin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SESN1 using anti-SESN1 antibody (A05559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SESN1 antigen affinity purified polyclonal antibody (Catalog # A05559-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SESN1 at approximately 56 kDa. The expected band size for SESN1 is at 56,65-75 kDa.
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