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SESN1 Antibody (YA2739, Rabbit)

HY-P82994
MedChem Express
TargetSESN1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SESN1 Antibody (YA2739, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID27244
  • Target name
    SESN1
  • Target description
    sestrin 1
  • Target synonyms
    PA26, SEST1, sestrin-1, p53 activated gene 26, p53 regulated PA26 nuclear protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SESN1 Antibody (YA2739) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2739), targeting SESN1, with a predicted molecular weight of 57 kDa (observed band size: 70 kDa). SESN1 Antibody (YA2739) can be used for WB, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SESN1 using anti-SESN1 antibody (A05559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SESN1 antigen affinity purified polyclonal antibody (Catalog # A05559-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SESN1 at approximately 56 kDa. The expected band size for SESN1 is at 56,65-75 kDa.
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