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Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Anti-SF3A3 Antibody

HPA032054
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSF3A3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SF3A3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10946
  • Target name
    SF3A3
  • Target description
    splicing factor 3a subunit 3
  • Target synonyms
    PRP9, PRPF9, SAP61, SF3a60, splicing factor 3A subunit 3, SAP 61, pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, splicing factor 3a, subunit 3, 60kD, splicing factor 3a, subunit 3, 60kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12874
  • Protein Name
    Splicing factor 3A subunit 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody (Catalog # A10671-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.
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