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SF3A3 / SF3a60 Antibody

LS-C831452
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetSF3A3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SF3A3 / SF3a60 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10946
  • Target name
    SF3A3
  • Target description
    splicing factor 3a subunit 3
  • Target synonyms
    PRP9, PRPF9, SAP61, SF3a60, splicing factor 3A subunit 3, SAP 61, pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, splicing factor 3a, subunit 3, 60kD, splicing factor 3a, subunit 3, 60kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody (Catalog # A10671-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.
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