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Immunohistochemical staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.
Immunohistochemical staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.
Immunohistochemical staining of human heart muscle shows distinct positivity of intercalated discs in myocytes.

Anti-SGCA Antibody

HPA007537
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSGCA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SGCA Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6442
  • Target name
    SGCA
  • Target description
    sarcoglycan alpha
  • Target synonyms
    50DAG, ADL, DAG2, DMDA2, LGMD2D, LGMDR3, SCARMD1, adhalin, alpha-sarcoglycan, 50 kDa dystrophin-associated glycoprotein, 50kD DAG, alpha-SG, dystroglycan-2, limb girdle muscular dystrophy 2D, sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16586
  • Protein Name
    Alpha-sarcoglycan
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SGCA using anti-SGCA antibody (PB9503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa.
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